[Na] and [K] dependence of the Na/K pump current-voltage relationship in guinea pig ventricular myocytes.
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چکیده
منابع مشابه
[Na] and [K] dependence of the Na/K pump current-voltage relationship in guinea pig ventricular myocytes
Na/K pump current was determined between -140 and +60 mV as steady-state, strophanthidin-sensitive, whole-cell current in guinea pig ventricular myocytes, voltage-clamped and internally dialyzed via wide-tipped pipettes. Solutions were designed to minimize all other components of membrane current. A device for exchanging the solution inside the pipette permitted investigation of Na/K pump curre...
متن کاملSteady-state current-voltage relationship of the Na/K pump in guinea pig ventricular myocytes
Whole-cell currents were recorded in guinea pig ventricular myocytes at approximately 36 degrees C before, during, and after exposure to maximally effective concentrations of strophanthidin, a cardiotonic steroid and specific inhibitor of the Na/K pump. Wide-tipped pipettes, in combination with a device for exchanging the solution inside the pipette, afforded reasonable control of the ionic com...
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To examine effects of cytosolic Na+, K+, and Cs+ on the voltage dependence of the Na+-K+ pump, we measured Na+-K+ pump current (Ip) of ventricular myocytes voltage-clamped at potentials (Vm) from 100 to +60 mV. Superfusates were designed to eliminate voltage dependence at extracellular pump sites. The cytosolic compartment of myocytes was perfused with patch pipette solutions with a Na+ concent...
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Binding of cardiac glycosides to the Na+,K+-dependent ATPase has been shown to occur at both high- and low-affinity sites. However, recent reports suggest that glycoside-induced inhibition of electrogenic Na-K pump current occurs with simple first-order binding kinetics at relatively low-affinity sites. This implies that high-affinity binding sites have little to do with Na-K pump inhibition du...
متن کاملChloride Current in Guinea Pig Ventricular Myocytes
Cardiac ventricular myocytes from several species, including the guinea pig, possess a cAMP-dependent protein kinase A (PKA)-activated Clchannel. In the present study, the properties of a protein kinase C (PKC)-activated Clcurrent were studied in isolated guinea pig ventricular myocytes using the whole-cell arrangement of the patch-clamp technique. Intracellular dialysis of ventricular cells wi...
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ژورنال
عنوان ژورنال: Journal of General Physiology
سال: 1989
ISSN: 0022-1295,1540-7748
DOI: 10.1085/jgp.94.3.539